Invitrogen™ Human IL-9 ELISA Kit

Description

ELISA

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.

Specifications

• Accession Number: P15248
• Assay Range: 3,1 to 200 pg/mL
• Assay Sensitivity: 0.5 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3578
• Gene Symbol: Il9
• Interassay CV: 4.8%
• Intraassay CV: 5.3
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Sample diluent, Biotinylated Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate, SAV-HRP, TMB, Stop solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: IL-9
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins