Invitrogen™ IL-9 Monoclonal Antibody (MH9A4), eFluor™ 660, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 50709742

Catalog No. 50-709-742

Description

Description: The monoclonal antibody MH9A4 recognizes interleukin-9 (IL-9), a proinflammatory cytokine historically believed to be involved in type 2 immune responses. Recent evidence suggests IL-9 may instead be secreted by a distinct T-helper lineage called Th9. These cells can be derived from Th2 cells with TGF beta or differentiated directly from naive CD4+ T cells with TGF beta and IL-4. IL-9 is a member of the common cytokine receptor gamma chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. Staining with the MH9A4 antibody is not blocked by the MH9D1 antibody, suggesting that the two antibodies bind to unique epitopes. Applications Reported: This MH9A4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MH9A4 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 em...

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.

Specifications

• Antigen: IL-9
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P15248
• Gene Symbols: Il9
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3578
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: MH9A4
• Conjugate: eFluor 660
• Gene: Il9
• Gene Alias: cytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; IL9; IL-9; ILN; Interleukin; interleukin 9; Interleukin9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ