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Invitrogen™ IL5RA Polyclonal Antibody

Catalog No. PIPA124792
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25 μg
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Catalog No. Quantity
PIPA124792 25 μg
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Catalog No. PIPA124792 Supplier Invitrogen™ Supplier No. PA124792

Description

Goat Polyclonal Antibody

Recommended positive controls: human blood-derived granulocytes. The Neutralization Dose50 (ND50) for anti-human IL5RA is approximately 1-3 μg/mL in the presence of 0.5 ng/mL of recombinant human IL-5 using the human TF-1 cell line. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cell surface IL5RA mediated IL5 response on a responsive cell line, at a specific IL5 concentration. The exact concentration of antibody required to neutralize the human cell surface IL5RA mediated bioactivity is dependent on the IL5 concentration as well as on the number of IL5RA present on the cell surface (a function of the cell type and culture conditions).

Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cell replacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membrane-proximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear proto-oncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IL5RA
Applications Flow Cytometry, Inhibition Assays, Neutralization, Western Blot
Classification Polyclonal
Concentration 0.1 mg/mL
Conjugate Unconjugated
Formulation PBS with 5% trehalose and no preservative; pH 7.4
Gene IL5RA
Gene Accession No. Q01344
Gene Alias CD125; CD125 antigen; CDw125; HSIL5R3; IL-5 receptor alpha chain; IL-5 receptor subunit alpha; Il5r; IL-5R subunit alpha; Il5ra; IL-5RA; IL-5R-alpha; interleukin 5 receptor subunit alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor subunit alpha
Gene Symbols IL5RA
Host Species Goat
Immunogen Purified recombinant human IL-5 soluble receptor alpha, expressed in insect Sf 21 cells.
Purification Method Antigen affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3568
Target Species Human
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG
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Safety and Handling

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