Cytiva Hot Start Master Mix

Description

A ready-to-use 2X mastermix providing robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success

• Convenient, ready-to-use mix saves time and reduces experimental variability
• For any standard PCR reaction, such as multiplex PCR, and reactions exhibiting nonspecific amplification or primer-dimer formation
• Primer sequestration effectively reduces any nonspecific priming or primer-dimer formation
• Recombinant activator protein avoids the risk of contamination from a mammalian source associated with hot start antibodies
• Avoids the risk of contamination from a mammalian source associated with hot start antibodies
• Quick hot start activation preserves sample integrity by avoiding extensive precycling heat treatment

Specifications

• Content And Storage: 2X Concentrate solution of 20mM Tris HCl (pH 8.6), 100mM KCl, 3mM MgCl2, 0.4mM dNTPs (dATP, dCTP, dGTP, dTTP), 50U/mL Taq DNA Polymerase, hot start activator protein, stabilizers. Store at -20°C
• Description: Hot Start Master Mix is a 2X premixed formulation that can effectively reduce nonspecific priming and primer-dimer formation during PCR.
• Form: Solution
• Format: Solution
• Quantity: 100 Reactions
• For Use With (Application): Standard PCR
• No. of Reactions: 100
• Volume: 50μL