Cytiva IMAC Sepharose™ 6 Fast Flow Affinity Media

Description

Used for purifying proteins and peptides that have an affinity for metal ions. Cytiva IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.

• Uncharged IMAC resin allows possibility to charge with metal ion of choice for optimized selectivity
• Used for ions such as histidine-tagged proteins but also untagged recombinant or native proteins
• High protein binding capacity
• Coupling chemistry and novel ligand designed to give minimal ligand leakage
• Available HiTrap™ and HiPrep™ pre-packed formats for easy scale-up and process development

 

Specifications

• Quantity: 100 mL
• For Use With (Application): For Purifying Proteins and Peptides That Have an Affinity for Metal Ions
• Stability: Stable in commonly used aqueous Buffers: 0.01 M HCl, 1 M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-Propanol
• Storage Requirements: 4°C to 30°C
• Product Type: IMAC Sepharose 6 Fast Flow Media
• Flow Rate: 250 to 400 cm/Hoμr
• Particle Size: 45 to 165 μm
• Height (English) Bed: 9.84 in.
• Height (Metric) Bed: 25 cm
• Max. Pressure: 100 kPa
• Matrix: 6% Cross-Linked Agarose
• pH Range: 3 to 12
• Storage Buffer: 20% Ethanol