Invitrogen™ Image-iT™ Low Density Lipoprotein Uptake Kit, pHrodo™ Red

Catalog No.	Quantity
I34360	1 kit

Catalog No. I34360 Supplier Invitrogen™ Supplier No. I34360

Description

The Image-iT Low Density Lipoprotein Uptake Kit - pHrodo Red includes pHrodo Red dye-labeled LDL (also available separately), unlabeled LDL, heparin, BSA, and assay buffer.

The kit was designed to specifically detect binding and uptake of LDL through the LDL receptor internalization pathway, allowing maximum control and flexibility in the experimental design. It can be used with fluorescence microscopy, flow cytometry, cell sorting, high-throughput analysis, and high content analysis.

• pHrodo Red-LDL is detectable with RFP/TRITC filter set (absorption peak: ∼560 nm, emission peak: ∼585 nm)
• pHrodo Red-LDL is multiplexable with green, deep red, violet, and blue emission dyes, such as GFP, DAPI, Hoechst, HCS LipidTOX, and LysoTracker Deep Red
• No-wash protocol is possible, allowing dynamic measurement of LDL uptake and trafficking

pHrodo Red- LDL is dimly fluorescent at neutral pH outside of cells, but fluoresces brightly after endocytosis. This feature allows for a no-wash protocol when using pHrodo Red-LDL, enabling kinetic measurement of LDL uptake, endosomal maturation, and trafficking in cells.

Two controls are included in the kit: unlabeled LDL as an optional pretreatment to occlude cell surface receptors in advance of probing with the labeled construct, and heparin which will chelate the LDL in the solution phase outside of the cells, preventing binding and uptake. When cells are labeled in the absence of these pretreatment controls, a fluorescent signal indicates specific LDL receptor-mediated endocytosis.

Specifications

• Content And Storage:
  • 1 x 200 μL pHrodo Red-LDL, 1 mg/mL
  • 1 x 200 μL LDL (unlabeled), 2.5 mg/mL
  • 1 x 50 mL LDL assay buffer, 1 X
  • 1 vial heparin, 5 mg/mL
  • 1 x 150 mg BSA
  • Store in refrigerator (2°C to 8°C) and protect from light.
• Product Type: Lipoprotein Uptake Kit
• Dye Type: pH-Sensitive Dyes
• Product Line: Molecular Probes
• Quantity: 1 kit
• Shipping Condition: Wet Ice

Frequently Asked Questions (FAQs)

Is the LDL in pHrodo Red-LDL acetylated or oxidized?

This product does not contain acetylated or oxidized LDL.

Why is fixation not recommended for cells stained with pHrodo Red-LDL?

pHrodo Red and Green dyes are pH-sensitive dyes. They should only be used on live cells to detect pH of cellular compartments. Upon fixation of the cells, the cell membrane is compromised and you are only measuring the pH of the buffer the samples are held in.

How is the pHrodo Red dye bound to the LDL in pHrodo Red-LDL?

pHrodo Red dye is covalently bound to the LDL.

For Research Use Only. Not for use in diagnostic procedures.