Invitrogen™ Importin beta-1 Monoclonal Antibody (3E9)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA3070

Catalog No. MA3070

Description

MA3-070 detects Importin beta 1 from human, canine, bovine, rat and mouse tissues as well as porcine cell lysates. Importin beta 1 is also known as NTF97, Nuclear Transport Factor p97, and karyopherin beta 1. This antibody does not detect bacterially expressed recombinant NTF97. MA3-070 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects a single ~97 kDa protein representing NTF97 in MDBK cell homogenate. Immunofluorescence staining of NTF97 in MDBK cells with MA3-070 results in the labeling of both the nuclear envelope (NE) and the cytoplasm and shows discontinuous, punctate staining along the rim of the NE. This antibody can inhibit import of nuclear localization sequence-containing proteins in permeabilized cells. The MA3-070 antigen is purified NTF97 from bovine erythrocytes.

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Specifications

• Antigen: Importin beta-1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: P52296, P70168, Q14974
• Gene Symbols: KPNB1
• Immunogen: Purified NTF97 from bovine erythrocytes.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Bovine, Pig
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Inhibition Assays, Immunoprecipitation, Western Blot, RNA Immunoprecipitation, Immunocytochemistry
• Clone: 3E9
• Conjugate: Unconjugated
• Gene: KPNB1
• Gene Alias: AA409963; HGNC:6400; IMB1; Impnb; importin 1; importin 90; importin beta; importin beta-1 subunit; importin subunit beta-1; Importin-90; IPO1; IPOB; KapB1; karyopherin (importin) beta 1; karyopherin beta 1; karyopherin subunit beta 1; karyopherin subunit beta-1; karyopherin,beta 1; Kpnb1; MGC2155; MGC2156; MGC2157; NTF97; Nuclear factor p97; pore targeting complex 97 kDa subunit; PTAC97; SCG
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100518449, 16211, 24917, 3837, 491042, 507804
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid