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Invitrogen™ eBioscience™ Indo-1 AM Calcium Sensor Dye Non-distribution product as customer accommodation.

Membrane-permeable dye used to determine changes in calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Once Indo-1 enters the cell, esterases cleave the AM group yielding a membrane-impermeable dye.

Manufacturer:  Invitrogen™ 65085639

Catalog No. 50-169-56


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Description

Description

  • Unbound Indo-1 has a peak emission at 475nm
  • Upon binding calcium, peak emission shifts down to 410nm
  • In flow cytometry this can be measured over time and can be represented as a ratio of the two emission wavelengths
  • To optimize the ratio between the two emissions, unbound Indo-1 fluorescence should be collected using a filter above 485nm; bound Indo-1 fluorescence should be collected using a filter below 400nm
  • Because the emission profile of Indo-1 is broad, multicolor flow analysis using eFluor ™ 450, eVolve™ 605 or eVolve™ 655 is not possible, however fluorochrome conjugated antibodies utilizing the 488nm or 633 nm are quite compatible with Indo-1
  • Molecular weight: 1009.91g/mol
  • Peak excitation: 346nm
  • Peak emission: 475nm
  • Reconstitute in high-quality, freshly opened DMSO
  • Recommended concentration: 1 mM
  • Once reconstituted, store at -20°C with dessicant and use within a short period of time. Avoid freeze-thawing
Specifications

Specifications

−20°C. Protect from light and moisture.
Lyophilized
>90% by HPLC
Yellow
Flow Cytometric Analysis, Microscopy, Immunohistochemical Staining
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