Invitrogen™ Influenza A H1N1 HA (A/California/04/2009) His-tag Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (400 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin. The distinct antigenic properties of the new swine influenza virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups.

Specifications

• For Use With (Application): Control
• Format: Lyophilized
• Formulation: 20 mM tris with <0.1 μg/mL BSA, 0.01% mannitol, 0.01% Tween 80, 10% glycerol, 150 mM NaCl, 5-8% trehalose and no preservative; pH 7.5
• Molecular Weight (g/mol): 58.7 kDa
• Name: Influenza A H1N1 HA (A/California/04/2009) His-tag
• Purification Method: SDS-PAGE
• Quantity: 100 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: flu hemagglutinin; H1N1 HA; H1N1 hemagglutinin; Influenza H1N1; influenza hemagglutinin
• Common Name: Influenza A H1N1 HA
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Influenza A virus H1N1 (A/California/04/2009) hemagglutinin, amino acids His36-Lys469 (Accession # ACP41107.1) with an N-terminal a vasodilator-stimulated phosphoprotein tetramerization domain and a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: Baculovirus
• Form: Lyophilized
• Purity or Quality Grade: >90% as determined by SDS-PAGE.