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Description
INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from human tissue lysates, designed to streamline the western blotting procedure by eliminating sample acquisition, preparation, SDS-PAGE, and electro-blotting steps.
- Applications: Western Blot
- Species Reactivity: Human
- Storage: Store at room temperature
- Guarantee: 1 Year from date of receipt
- Buffer Composition: Tissues are lysed in a buffer containing 10 mM Tris (pH 8.0), 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi, Protease Inhibitor Cocktail (PIC), and PMSF. Lysates are resuspended in 2X SDS sample buffer containing 120 mM Tris-HCl (pH 6.8), 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, and 0.4% β-mercaptoethanol.
Specifications
Specifications
| For Use With (Application) | Western Blot |
| Quantity | 1 Membrane |
| Content And Storage | Store at room temperature. |
| Product Line | Novus |
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