MilliporeSigma™ Integrin β1, Rat, Alexa Fluor 647, Clone: MB1.2,

Catalog No.	Quantity	Conjugate
MAB1997AF64	100 μL	Alexa Fluor 647

Catalog No. MAB1997AF64 Supplier MilliporeSigma™ Supplier No. MAB1997AF647

Description

Rat Monoclonal Antibody

Integrin beta-1 (UniProt P09055; also known as CD29, Fibronectin receptor subunit beta, GpIIa, VLA-1/2/3/4/5/6 subunit beta) is encoded by the Itgb1 (also known as Fnrb) gene (Gene ID 16412) in murine species. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. Integrin beta-1 is a 130kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

Specifications

• Antigen: Integrin β1
• Applications: Immunocytochemistry
• Classification: Monoclonal
• Clone: MB1.2
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Alexa Fluor 647
• Formulation: Purified rat IgG2a Alexa Fluor™ 647 conjugate in buffer containing PBS, 1.5% BSA with 0.05% Sodium Azide.
• Gene: Itgb1, Fnrb
• Gene Accession No.: P09055
• Host Species: Rat
• Immunogen: Mouse Integrin beta-1 (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132).
• Purification Method: Protein G Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Epigenetics & Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_034708
• Test Specificity: The specificity of MB1.2 was determined by its binding to antigen, and the ability of antiserum against the carboxyl terminal of VLA-beta 1 subunit to deplete antigens for MB1.2 in sequential immunoprecipitation experiments. MB1.2 had no effect on cell adhesion to matrix proteins (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132).
• Target Species: Mouse
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.