Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 46588782

Catalog No. 50-112-3857

Description

Description: The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha 4/ beta 7), which is a heterodimer of the the 154 kDa alpha 4 subunit and the 130 kDa beta 7 subunit. In mouse, the beta 7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion. Applications Reported: This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis. Applications Tested: This DATK32 (DATK-32) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5.

Integrin alpha 4 beta 7 (LPAM-1) Monoclonal antibody specifically detects Integrin alpha 4 beta 7 (LPAM-1) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: Integrin alpha 4 beta 7 (LPAM-1)
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P26011, Q00651
• Gene Symbols: Itga4, Itgb7
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16401, 16421
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: DATK32 (DATK-32)
• Conjugate: PerCP-eFluor 710
• Gene: Itga4
• Gene Alias: CD49 antigen-like family member D; CD49D; integrin alpha 4; integrin alpha-4; integrin alpha4B; Integrin alpha-IV; integrin beta 7; integrin beta-7; Integrin beta-P; Itga4; Itga4B; Itgb7; LPAM subunit alpha; Ly69; lymphocyte Peyer patch adhesion molecules subunit alpha; M290 IEL antigen; VLA; VLA 4; VLA-4 receptor, alpha 4 subunit; VLA4 subunit alpha; VLA-4 subunit alpha
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.