Integrin beta1 (CD29), Rat anti-Human, Clone: mAb13, Millipore Sigma™

Rat Monoclonal Antibody

Supplier:  MilliporeSigma MABT82125UL

Catalog No. MABT82125UL

Description

Anti-Integrin beta1 (CD29), clone mAb13, Cat. No. MABT821, is a rat monoclonal antibody that detects integrin beta-1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Inhibition studies.

Integrin beta-1 (UniProt: P05556; also known as Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12) gene (Gene ID: 3688) in human. Integrin beta-1 is a single-pass type I membrane protein that associates with one of the alpha subunits to form receptor for fibronectin. Integrin beta-1 integrin recognizes the sequence R-G-D in a wide array of ligands. Five isoforms of integrin beta-1 have been reported that are produced by alternative splicing. Integrin beta-1 is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved off. The mature form has an extracellular domain (aa 21-728), a helical domain (aa 729-751) and a cytoplasmic region (aa 752-798). Isoform 1 of integrin beta-1 is widely expressed and other isoforms are generally co-expressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4 is selectively expressed in peripheral T-cells and isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle). Isoform 2 is reported to interfere with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro).

Specifications

• Antigen: Integrin beta1 (CD29)
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified rat monoclonal antibody IgG2a in PBS without preservatives.
• Gene Symbols: ITGB1;FNRB;MDF2;MSK12
• Immunogen: Fibronectin receptor (alpha V beta 1) purified from human placenta.
• Quantity: 25 μL
• Research Discipline: Cell Structure
• Test Specificity: Clone mAb13 specifically detects beta 1 subunit of human integrin. It targets an epitope within 12 amino acids from the N-terminal region.
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Isotype: IgG2a κ

• Applications: Immunocytochemistry, Immunoprecipitation, Inhibition Assays
• Clone: mAb13
• Dilution: Inhibition Analysis: A representative lot blocked cell attachment in Inhibition studies (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43). Immunocytochemistry Analysis: A representative lot detected Integrin beta1 (CD29) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43). Immunoprecipitation Analysis: A representative lot detected Integrin beta1 (CD29) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75). Inhibition Analysis: A representative lot blocked  HT-1080 cells adhesion to fibronectin coated plates  (Courtesy of Dr. Kenneth M. Yamada, M.D., Ph.D., NIDCR, National Institutes of Health, Bethesda, MD USA).
• Gene Alias: Fibronectin receptor subunit beta;Glycoprotein IIa;GPIIA;VLA-4 subunit beta;CD29
• Host Species: Rat
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified