Invitrogen™ Human IFN-gamma Recombinant Protein

Catalog No.	Quantity
PIRP8607	100 μg

Catalog No. PIRP8607 Supplier Invitrogen™ Supplier No. RP8607

Description

Recombinant Protein

Recombinant human IFN-gamma is a non-glycosylated protein containing 144 amino acids. Reconstitute using sterile water at 0.1 mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Specifications

• Accession Number: P01579
• Concentration: 0.1 mg/mL
• For Use With (Application): Control, Bioactivity
• Formulation: 10mM sodium phosphate with 50mM NaCl and no preservative; pH 7.5
• Gene ID (Entrez): 3458
• Molecular Weight (g/mol): 16.9 kDa
• Name: Human IFN-gamma
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 100 μg
• Source: E. Coli
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: ≤0.1 EU/μg
• Gene Alias: BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g
• Common Name: IFN gamma
• Gene Symbol: IFNG
• Biological Activity: ED50 <0.75 ng/mL; determined in Viral challenge assay using EMC virus on A549 cells
• Product Type: Protein
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: ED50 <0.75 ng/mL; determined in Viral challenge assay using EMC virus on A549 cells
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: ≥95%

Safety and Handling

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