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Invitrogen™ AccuPrime™ Pfx DNA Polymerase

AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.

Supplier:  Invitrogen™ 12344024


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Catalog No. 12-344-024

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Includes: AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.



AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum™ anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum™ Pfx alone.

With AccuPrime™ Pfx DNA Polymerase you will:
• Achieve greater than 26 times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimization

Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime™ Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.

Order Info

Approved for shipment on Wet or Dry Ice



• AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL)
• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)

Store at -20°C.
Wet or Dry Ice
200 Reactions
Separate Components
100 μL
AccuPrime Pfx DNA Polymerase
200 Reactions
Hot-start PCR, High-fidelity PCR
Built-In Hot Start
12 kb or less
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WARNING: Cancer -


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