DNA Polymerase I Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

DNA polymerase with 5'3' and 3'5' exodeoxyribonuclease activities

Manufacturer: Invitrogen 18010017

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Catalog No. 18-010-017

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    Specifications

    Product Type Polymerase (1), DNA
    Includes 1000 units of DNA polymerase in a storage buffer of 50mM potassium phosphate (pH 7.0), 100mM KCl, 1mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/μL
    For Use With (Application) Cloning, Restriction Enzyme Cloning
    Quantity 250 units

    Performance and quality testing
    Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.

    Unit definition

    One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.

    Unit reaction conditions

    50mM potassium phosphate (pH 7.0), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80µg/mL template/primer, 32µM dTTP, 69nM [3H]dTTP, and enzyme in 100µL for 30 min at 37°C. Also incorporates biotinylated nucleotides.

    • Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs
    • Source: purified from E. coli expressing the DNA Polymerase I gene on a plasmid

    Cloning, Restriction Enzyme Cloning

  • Description & Specifications

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