Invitrogen™ Dynabeads™ M-280 Streptavidin  

For solation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets

Includes: Dynabeads (2.8µm) with covalently coupled recombinant streptavidin. Supplied in PBS, pH 7.4 with 0.1% BSA and 0.02% sodium azide added as a preservative

Catalog Number	Mfr. No.	Quantity
11-205-D	Invitrogen™ 11205D	2mL
11-206-D	Invitrogen™ 11206D	10mL
60-210	Invitrogen™ 60210	100mL

Description

Dynabeads are used on more than 25,000 routine IVD instruments worldwide and are known for exceptional reproducibility (both within and between batches) and automation ability. The strong binding affinity of streptavidin-biotin interaction (K_d=10^-15) is used in vast number of applications.

• Direct and fast isolation of any biotinylated molecule
• Low-charged and neutral beads, optimal for binding of DNA fragments, proteins, peptides, and antibodies
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Biomagnetic protocols are easily adapted to automated platforms
• High batch-to-batch reproducibility, securingconsistent results in application
• Bead Diameter: 2.8 µm
• Concentration: 10mg/mL
• High throughput compatible
• Sample Type: Any Sample Type

• Diameter: 2.8°m, with monolayer, not a multilayer, of recombinant streptavidin covalently coupled to surface and further blocked with BSA
• Show rapid liquid-phase reaction kinetics.
• Key applications: Preparing 2 to 5kb samples for mate pair library sequencing, isolation of RNA and DNA binding proteins, immobilization of large DNA fragments, purifying sequencing products, specific capture of nucleic acids
• Dynabeads™ are used on more than 25,000 routine IVD instruments worldwide

One mg of Dynabeads M-280 Streptavidin typically binds:

• 650 to 900 pmoles free biotin
• ~200 pmol biotinylated peptides
• ~10µg biotinylated IgG
• ~10µg ds-DNA
• ~200 pmol ss-oligonucleotides

Antibody Purification, ChIP-on-Chip, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), DNA and RNA Purification and Analysis, DNA Extraction, Immunoprecipitation, Phage Display, Protein and Peptide Purification, Protein Complex Isolation and Analysis, Protein Complex Pull-Down, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis, RNAi, Epigenetics and Non-Coding RNA Research, Sanger Sequencing, Sequencing, Sequencing Reaction Purification

Order Info

Shipping Condition: Room Temperature

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Safety and Handling

ShelfLife : 36 months from date of manufacture

For Research Use Only. Not for use in diagnostic procedures.