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Invitrogen™ Platinum™ Taq DNA Polymerase High Fidelity Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Ideal for amplification of DNA fragments when high yields and robust amplification are required

Manufacturer:  Invitrogen™ 11304102

Catalog No. 11-304-102


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Includes: Platinum Taq DNA Polymerase High Fidelity (1000µL, at 5U/µL) is supplied with a bottle (50mL) of 10X High Fidelity Buffer (600mM Tris-SO4 (pH 8.9), 180mM (NH4)2SO4), and a bottle (25mL) of 50mM MgSO4.

Description

Description

High fidelity is provided by a mixture of PlatinumTaq DNA Polymerase and the proofreading (3'-5' exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum automatic “hot-start” technology.

  • Fidelity: greater than six times higher fidelity than Taq DNA polymerase
  • Amplicon size: amplification of fragments up to 15kb
  • Convenience: room temperature reaction assembly
  • Applications: amplification of DNA from complex genomic, viral, and plasmid templates and RT-PCR
Unit definition:
One unit of PlatinumTaq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.

High Fidelity PCR, Hot Start PCR, Long Fragment PCR, PCR, PCR and Real-Time PCR, Sanger Sequencing, Sanger Sequencing Technology and Accessories, Sequencing

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Order Info

Shipping Condition: Dry ice

Specifications

Specifications

3' - 5', 5' - 3'
Low
5000.0
Platinum™ Taq DNA Polymerase High Fidelity
20 kb or less
6 X
Platinum Taq DNA Polymerase High Fidelity (1000μL, at 5U/μL) is supplied with a bottle (50mL) of 10X High Fidelity Buffer (600mM Tris-SO4 (pH 8.9), 180mM (NH4)2SO4), and a bottle (25mL) of 50mM MgSO4.
3'-A, Mixed
5000 Reactions
Store at -30°C to -10°C.
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For Research Use Only. Not for use in diagnostic procedures.