Invitrogen™ SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit

Description

Includes

100μL SuperScript III RT/Platinum Taq Mix; 2 x 1.25mL 2X SYBR Green Reaction Mix; 1mL Magnesium Sulfate (50mM); 100μL ROX Reference Dye (25μM); 300μL 20X Bovine Serum Albumin (1mg/mL)

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

The SuperScript™ III Platinum™ One-Step qRT-PCR Kit provides sensitive, specific detection with fluorogenic primers and probes, while the SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit offers the ease and convenience of SYBR™ Green I detection.

• Kit combines the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format
• The SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit offers the ease and convenience of SYBR™ Green I detection
• SuperScript™ III RT for higher temperature cDNA synthesis for greater success with difficult RNA secondary structure
• Platinum™ Taq DNA Polymerase with hot-start technology for improved specificity
• LUX™ Fluorogenic Primers or dual-labeled fluorogenic probes for superior detection performance
• SYBR™ Green I dye for easy and convenient detection
• Validated performance on multiple real-time instrument platforms, including rotor-based systems
• Store all components at -20°C (-80°C for long-term storage)
• ROX Reference Dye must be stored in the dark

Order Info

Shipping Conditions: Dry Ice

Specifications

• Content And Storage: • 500 μL SuperScript™ III RT/Platinum™ Taq Mix
  • 12.5 mL 2X SYBR™ Green Reaction Mix
  • 2 × 1 mL Magnesium Sulfate (50 mM)
  • 500 μL ROX Reference Dye (25 μM)
  • 1.3 mL 20X Bovine Serum Albumin (1 mg/mL)
  
  Store all components at -20°C (-80°C for long-term storage). ROX Reference Dye must be stored in the dark.
• Detection Method: SYBR
• PCR Method: 1-step RT-qPCR
• Polymerase: Taq DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
• Product Line: Platinum™, SYBR™, SuperScript™
• Product Type: One-Step qRT-PCR Kit
• Quantity: 500 reactions
• Shipping Condition: Dry Ice
• Sufficient For: 500 Reactions
• For Use With (Application): Real Time PCR (qPCR)
• No. of Reactions: 500 Reactions
• Sample Type: RNA

For Research Use Only. Not for use in diagnostic procedures.