Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit

Description

The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli.

The PureLink™ HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350 μg plasmid DNA from 15–25 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients.

Advantages of the PureLink™ HiPure Plasmid Midiprep Kit include:

• Labor and time saving—no need for extra steps to remove endotoxin and other contaminants
• High-purity, low-endotoxin product—pure enough for mammalian cell transfection
• Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification

The PureLink™ HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. The resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal. Plasmid preparations can typically be completed in less than 2 hours.

Contaminant-free DNA

Unlike cesium chloride gradient protocols, PureLink™ HiPure Plasmid Purification Systems do not use organic solvents, ethidium bromide, or cesium chloride, all of which are troublesome to work with and to dispose of. Typically, plasmid DNA prepared using the PureLink™ HiPure Plasmid Midiprep Kit will have an A₂₆₀/A₂₈₀ ratio of greater than 1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications. Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: • 100 mL Resuspension Buffer (R3)
  • 550 μL RNase A
  • 100 mL Lysis Buffer (L7)
  • 100 mL Precipitation Buffer (N3)
  • 250 mL Equilibration Buffer (EQ1)
  • 500 mL Wash Buffer (W8)
  • 125 mL Elution Buffer (E4)
  • 15 mL TE Buffer
  • 25 HiPure Columns
  • 5 Column Holders
  
  Store all components at room temperature.
• Format: Column
• Isolation Technology: Anion Exchange Resin
• Sample Type: Bacterial Culture
• Elution Volume: 5 mL
• Final Product Type: BAC DNA, Plasmid DNA
• For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• No. of Reactions: 25 Preps
• Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
• Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
• Product Line: PureLink™
• Product Type: Plasmid MidiPrep Kit
• Quantity: 25 Preps
• Purity: Transfection Grade
• Shipping Condition: Room Temperature
• Target: BAC DNA, Plasmid DNA
• System Type: PureLink™
• Test Time: 2 hr.
• Yield: 100 to 350 μg

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.