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Invitrogen™ PureLink™ PCR Purification Kit

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.

$129.00 - $594.00

Specifications

Format Kit
Run Time 10 min.
Shipping Condition Room Temperature
Isolation Technology Silica Spin Column
Sample Type PCR Products
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
K310001
Encompass_Preferred
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Invitrogen™
K310001
50 Preps
Each for $129.00
Edge
Add to Cart
 
K310002
Encompass_Preferred
View Documents
Invitrogen™
K310002
250 Preps
Each for $594.00
Add to Cart
 
Description

Description

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits. After PCR amplification, purification of the PCR amplicons is required for many common downstream applications, including enzyme digestion, ligation, sequencing, and labeling experiments, to prevent enzymes (polymerase, restriction enzyme) and reaction components (dNTPs, primers, buffers, salts) from carrying over, contaminating, and negatively influencing the downstream experiments.

Advantages of the PureLink PCR Purification Kit include:
• 4X binding capacity compared to Company Q; 40 μg for the PureLink kit versus 10 μg from other companies' kits
• No need for tedious sample pH adjustments necessary with other kits
• Convenient and rapid protocol, completed in less than 10 minutes
• Optional size-selection buffers included for selectively purifying 300–600 bp fragments or removing fragments <300 bp

The PureLink PCR Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts. Simply mix the PCR product with Binding Buffer and apply to the PureLink Spin Column. The dsDNA binds to the silica-based membrane in the column. Remove impurities by thorough washing with Wash Buffer. To purify the DNA, elute the dsDNA in low-salt Elution Buffer or water.The purified PCR product is suitable for automated fluorescent DNA sequencing, restriction enzyme digestion, and cloning.

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Kit
Room Temperature
PCR Products
10 min.
Silica Spin Column
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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