Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ SuperScript™ II Reverse Transcriptase

An improved version of SuperScript RT

$116.00 - $1416.00

Specifications

Concentration 200 U/μL
Final Product Type cDNA (First-Strand)
Format Stand-alone enzyme
GC-Rich PCR Performance High
PCR Method RT-PCR, RT-qPCR
View More Specs

Products 3
Catalog Number Mfr. No. Includes No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. Includes No. of Reactions Price Quantity & Availability  
18-064-022
Encompass
View Documents
Invitrogen™
18064022
SuperScript II Reverse Transcriptase (2,000 units total, at 200U/μL), 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and 100mM DTT 10 Reactions
Each for $116.00
Add to cart
 
18-064-071
Encompass
View Documents
Invitrogen™
18064071
200 Reactions
Each for $1,416.00
Add to cart
 
18-064-014
Encompass
View Documents
Invitrogen™
18064014
SuperScript II Reverse Transcriptase (10,000 units total, at 200U/μL), 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and 100mM DTT 50 Reactions
Each for $412.00
Add to cart
 
Description

Description

SuperScript II Reverse Transcriptase (RT) is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript II RT superior performance characteristics.
  • Yield: Reduced RNase H activity results in greater first-strand cDNA yields
  • Size of cDNA: Generation of RT-PCR products up to 12kb
  • Thermostability:Full activity at 42°C
  • Flexibility:Uses either total or poly(A)+ RNA
  • Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
  • Concentration: 200 U/μL
  • Unit definition:One unit of SuperScript II RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT)12-18 as template
  • primer Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)12-18, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C
  • Optimal reaction temperature: 42°C
  • Final product size: 12.3kb or less

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Wet ice

Specifications

Specifications

200 U/μL
Stand-alone enzyme
RT-PCR, RT-qPCR
Standard
RNA
RNA
Reverse Transcriptase
cDNA (First-Strand)
High
Separate components
Reverse Transcription
12.3 kb or Less
Reverse Transcription
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit