SuperScript™ III Reverse Transcriptase Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Proprietary mutant of SuperScript II RT

Manufacturer: Invitrogen 18080085

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Catalog No. 18-080-085

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    Specifications

    Concentration 200U/μL
    Format Frozen
    Includes SuperScript III Reverse Transcriptase (4 × 10,000 units total, at 200U/μL), (4 × 1mL) of 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and (4 × 500μL) of 100mM DTT
    Packaging Type Tube
    Quantity 200 reactions
    Storage Requirements Store at -20deg.C
    Temperature (English) Operating 122deg.F
    Temperature (Metric) Operating 50deg.C
    Test Sensitivity Medium

    SuperScript III Reverse Transcriptase (RT) is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples. Like SuperScript II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. SuperScript III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.

    • Thermostability: half-life of 220 minutes at 50°C
    • Yield: reduced RNase H activity for more full-length cDNA
    • Specificity: full activity at 50°C for increased specificity with gene-specific primers
      ·Applications—array labeling, cDNA libraries, RT-PCR, primer extension, 3' and 5' RACE
    • Source: Purified from E. coli expressing the pol gene of M-MLV, mutagenized to increase thermal stability and half-life and to reduce RNase H activity
    • Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
    • Concentration: 200U/µL

    • Unit definition: One unit of SuperScript III RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)·oligo(dT)12-18 as a template·primer
    • Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)12-18, 0.1mg/mL BSA, and enzyme in 50µL for 10 minutes at 37°C
    • Optimal reaction temperature: 50°C
    • Final product size: 12.3kb or less

    PCR and Real-Time PCR, Reverse Transcription, array labeling, cDNA libraries, primer extension, 3' and '5 RACE

  • Description & Specifications

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