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Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

Provides the high-temperature capability of SuperScript III Reverse Transcriptase (RT) in an optimized format for generating first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR)

$648.00 - $2880.00

Specifications

Content And Storage • 5X VILO Reaction Mix containing random primers, dNTPs, and MgCl2
• 10X SuperScript III Enzyme Blend containing SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor,and proprietary helper protein

Store in freezer (-5 to -30°C).
Concentration 10X
Detection Method Primer-Probe
Fidelity (vs. Taq) 10 X
Final Product Type cDNA (First-Strand)
View More Specs

Includes: 5X VILO Reaction Mix (200μL) and a 10X SuperScript III Enzyme Blend (100μL), which contains: SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, and a proprietary helper protein

Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
11-754-050
Encompass
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Invitrogen™
11754050
50 Reactions
Each for $648.00
Add to cart
 
11-754-250
Encompass
View Documents
Invitrogen™
11754250
250 Reactions
Each for $2,880.00
Add to cart
 
Description

Description

VILO is an acronym for variable input, linear output; and the SuperScript VILO cDNA Synthesis Kit provides the most consistent results for RT experiments, with unprecedented linearity across the broadest range of input material.

  • Using the SuperScript VILO cDNA Synthesis Kit This formulation provides enhanced cDNA synthesis efficiency and can be used with very low and very high amounts of input RNA (up to 2.5μg total RNA in a 20μL reaction), giving a linear response in message abundance as measured by qPCR. The 10X SuperScript Enzyme Mix includes SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, and a proprietary helper protein
  • SuperScript III RT SuperScript III RT is an engineered version of M-MLV RT with reduced RNase H activity and increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 42° to 60°C. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. RNaseOUT Recombinant Ribonuclease Inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination
  • Optimized buffer The 5X VILO Reaction Mix includes random primers, MgCl2, and dNTPs in a buffer formulation that has been optimized for qRT-PCR

  • Optimal reaction temperature: 42°C

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

Specifications

• 5X VILO Reaction Mix containing random primers, dNTPs, and MgCl2
• 10X SuperScript III Enzyme Blend containing SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor,and proprietary helper protein

Store in freezer (-5 to -30°C).
Primer-Probe
cDNA (First-Strand)
High
Master Mix
Reverse Transcription
10 kb or Less
Reverse Transcription
42°C
SuperScript™, VILO™
SuperScript™ III
Dry Ice
10X
10 X
Kit
RT-qPCR
Fast
RNA
RNA
20 μL
Random Primers
cDNA Synthesis Kit
Reduced
Real-Time Quantitative PCR (qPCR)
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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