Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Provides fast, efficient cloning and subsequent in vitro transcription/translation

Manufacturer:  Invitrogen™ 450640

Catalog No. 45-064-0


Add to cart

Includes: Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water

Description

Description

Includes the pCR™II-TOPO TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable cloning method, featuring a 5-minute cloning reaction and 3-step protocol, and yielding up to 95% recombinants. Resistant to Kanamycin (KanR) andZeocin™ (ZeoR)
Kit Options: TOPO TA Cloning Dual Promoter Kits
TOPO TA Cloning Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:

  • General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40)
  • High-efficiency cloning: TOP10 Electrocomp™ cells (Cat. Nos. K4660-01, K4660-40)
  • General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. Nos. K4620-01, K4620-40)
  • Fast growth: Mach1™ -T1R chemically competent E. coli (Cat. No. K4610-20)
  • Repressor/induction needs: TOP10F' cells (Cat. Nos. K4650-01, K4650-40)
  • Provide your own cells (Cat. Nos. 450640 and 452640)

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and SP6 promoters for efficient in vitro transcription
  • M13 forward and reverse primer sites for sequencing
  • 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
  • Kanamycin and ampicillin resistance for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants

Cloning, PCR Cloning

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

Kit, Cloning
Box 1: Topoisomerase I-activated pCRII-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water
Cloning, PCR Cloning
25 reactions
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.