Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells Non-distribution product as customer accommodation.

Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing

Manufacturer: Invitrogen 450159

 View more versions of this product

Catalog No. 45-015-9

  Check Availability
Add to Cart


    Product Type Kit, PCR Cloning
    Includes Box 1: Topoisomerase I-activated pCR4Blunt-TOPO vector, Salt solution, dNTPs, Control template, T3, T7, M13 forward, and M13 reverse primers, Control PCR primers, Sterile water
    For Use With (Application) Cloning, PCR Cloning
    Quantity 10 reactions

    Uses the pCR™4Blunt-TOPO™ vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. Include the tools necessary to clone and select recombinant vectors containing your PCR fragment of choice. Resistant to Ampicillin (AmpR) and Kanamycin (KanR).
    pCR4Blunt-TOPO Vector: Optimized for Sequencing
    The pCR4Blunt-TOPO vector has a minimized multiple cloning site and, thus, a shorter distance between the sequencing primer sites and the insert site (as little as 33 bp). So sequencing reactions produce less vector sequence and more insert sequence. The pCR4Blunt TOPO vector has sites for four common sequencing primers: M13 forward, M13 reverse, T7, and T3. The kits include an aliquot of each.
    pCR4Blunt-TOPO Clone Selection and Manipulation
    The pCR4Blunt-TOPO vector contains both ampicillin and kanamycin resistance markers and a LacZα-ccdB gene fusion for positive selection and blue/white screening. The vector's minimized multiple cloning site still includes flanking EcoRI sites for simplified excision of cloned PCR products and a unique Sse8387I site for generation of nested deletions prior to sequencing. T7 and T3 promoters are also present for in vitro transcription.
    Simplified TOPO-based Cloning
    Using TOPO cloning technology, there is no need for PCR primers containing specific sequences, post-PCR procedures, vector preparation, or other time-intensive DNA manipulation steps. Just add your PCR reaction straight to the provided topoisomerase-charged vector, incubate 5 minutes, and transform E. coli competent cells.
    Efficient Cloning
    With up to 95% of clones carrying the desired insert, you can screen fewer clones, which saves time and money. The pCR4Blunt-TOPO vector has no overhangs for efficient ligation of PCR products created by proofreading thermostable polymerases that leave blunt-ended PCR products.
    The Most Widely Used Cloning Kit
    When it comes to cloning, TOPO cloning technology has been a reliable partner for thousands of scientists for over ten years. Fast, simple-to-use, and efficient, TOPO cloning has been applied to many different vectors for a wide array of applications.
    Kit Options: Zero Blunt TOPO PCR Cloning Kits for Sequencing
    Zero Blunt TOPO PCR Cloning kits for sequencing can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:

    • General cloning: TOP10 cells (Cat. Nos. K2875-J10, K2875-20, K2875-40)
    • High-efficiency cloning: TOP10 Electrocomp™ cells (Cat. Nos. K2880-20, K2880-40)
    • General cloning, bacteriophage T1 resistance: DH5α-T1R (Cat. No. K2895-20)
    • Fast growth: Mach1™-T1R chemically competent E. coli (Cat. No. K2835-20)
    • Provide your own cells (Cat. Nos. 450159 and 450031)

    • Fast and easy: go from PCR to clones in just three steps, including a 5-minute cloning reaction
    • Efficient: obtain up to 95% clones with correct insert
    • Proven: enabling reliable performance for over a decade with over 20,000 citations
    • Simple: no ligase, post-PCR procedures, or PCR primers containing specific sequences required
    • Accurate: for use with high fidelity DNA polymerases, no need for A-overhang on PCR product
    • Antibiotic resistance: Ampicillin (AmpR), Kanamycin (KanR)

    Cloning, PCR Cloning

  • Description & Specifications

Related Products

Product Content Feedback

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit