Involucrin Mouse, Clone: SPM259, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP234354F	Novus Biologicals NBP2343540.02MG	0.02 mg
NBP234354A	Novus Biologicals NBP2343540.1MG	0.1 mg
NBP234354B	Novus Biologicals NBP2343540.2MG	0.2 mg

Description

Involucrin Monoclonal specifically detects Involucrin in Human, Porcine, Canine, Primate, Monkey, Mouse (Negative) samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Involucrin
• Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 1-2ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Pig, Canine, Primate, Mouse (Negative)
• Gene Accession No.: P07476
• Gene ID (Entrez): 3713
• Immunogen: Purified involucrin from human keratinocytes
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: SPM259
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Extracellular Matrix
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: involucrin
• Gene Symbols: IVL
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ∼115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.