Invitrogen™ IRE1 alpha Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529800

Catalog No. PIPA529800

Description

Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (99%), Rat (98%), Zebrafish (83%), Japanese Medaka (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.

Specifications

• Antigen: IRE1 alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ERN1
• Gene Alias: 9030414B18Rik; AI225830; C85377; endoplasmic reticulum (ER) to nucleus signalling 1; endoplasmic reticulum to nucleus signaling 1; endoplasmic reticulum to nucleus signalling 1; endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signalling 1; ERN1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring 1 alpha; inositol-requiring enzyme 1; inositol-requiring protein 1; IRE1; IRE1a; IRE1a antibody; Ire1alpha; Ire1-alpha; Ire1p; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; RGD1559716; Serine/threonine-protein kinase; Serine/threonine-protein kinase/endoribonuclease IRE1; serine/threonine-protein kinase/endoribonuclease IRE1-like
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2081, 417414
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 0.5 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O75460
• Gene Symbols: ERN1
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 224 of Human IRE1 alpha.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Chicken
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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