Ire1 Polyclonal Antibody, Rockland™

Catalog No.	Quantity
50-199-2152	25 μL
50-199-2151	100 μg

Catalog No. 50-199-2152 Supplier Rockland Immunochemicals Supplier No. 600401HB9S

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity purified antibody is directed against mouse Ire1. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human.

IRE1 alpha is the endoplasmic reticulum to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. IRE1 alpha protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.

Specifications

• Antigen: Ire1
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Classification: Polyclonal
• Concentration: 0.97 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.01M potassium phosphate with 0.25M NaCl and 0.01% sodium azide; pH 7.2
• Gene: ERN1
• Gene Accession No.: O75460, Q9EQY0
• Gene Alias: 9030414B18Rik; AI225830; C85377; endoplasmic reticulum (ER) to nucleus signalling 1; endoplasmic reticulum to nucleus signaling 1; endoplasmic reticulum to nucleus signalling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signalling 1; Ern1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring 1 alpha; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE1; IRE1a; IRE1a antibody; Ire1alpha; Ire1-alpha; Ire1p; IRE1p antibody; LOC100357346; LOC417414; MGC163277; MGC163279; protein kinase/endoribonuclease; RGD1559716; Serine/threonine-protein kinase; Serine/threonine-protein kinase/endoribonuclease IRE1
• Gene Symbols: ERN1
• Host Species: Rabbit
• Quantity: 25 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 2081, 78943
• Target Species: Human, Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid