IRF8 Mouse anti-Human, PE, Clone: U31-644, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 566373

Catalog No. BDB566373

Description

The U31-644 monoclonal antibody specifically recognizes human Interferon regulatory factor 8 (IRF8, Interferon consensus sequence-binding protein, ICSBP or H-ICSBP). IRF8 is a ∽50kDa transcription factor that belongs to the interferon (IFN) regulatory factor family which consists of 9 members in humans and mice. IRF8 is variably expressed in hematopoietic cells including monocytes, macrophages, dendritic cells, and B cells. IRF8 has a conserved N-terminal DNA-binding domain (DBD) and a divergent C-terminal region that functions as the regulatory domain which specifies interactions between IRF8 and other IRF family members, transcription factors, or cofactors. IRF8 functions as a transcriptional activator or repressor depending on the formation of heterodimeric DNA binding complexes with these different partners. IRF8 binds to specific DNA motifs including IFN-stimulated response elements (ISRE) and regulates expression of genes including those that encode IFN-alpha (IFN-α), IFN-beta (IFN-β), and IFN-inducible MHC class I genes. It functions in innate and adaptive immunity by regulating the effector functions of monocytes/macrophages, B cells and dendritic cell subsets. IRF8 participates in the induction of Type I IFNs, TNF, IL-6, and IL-12 expression by leucocytes stimulated with ligands for Toll-like receptors and other pattern recognition receptors (PRRs). IRF8 is also involved in cell cycle regulations and apoptosis and may play a role in tumorigenesis.

R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.

Specifications

• Antigen: IRF8
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: IRF-8, IRF8, ICSBP, H-ICSBP, ICSBP1
• Immunogen: Human IRF8 Recombinant Protein
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human

• Clone: U31-644
• Conjugate: PE
• Format: Affinity Purified
• Host Species: Murine
• Isotype: Mouse IgG₁, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal