IRS1, p Ser307 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NBP178078

Catalog No. NBP178078

Description

IRS1 Polyclonal specifically detects IRS1 in Human samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: IRS1 (p Ser307)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: HIRS-1, insulin receptor substrate 1, IRS-1
• Host Species: Rabbit
• Molecular Weight of Antigen: 131.59 kDa
• Quantity: 0.1 mg
• Research Discipline: Autophagy, Cancer, Diabetes Research, Lipid and Metabolism, mTOR Pathway, Nutrient Sensing in the Brain, Phospho Specific, Signal Transduction
• Gene ID (Entrez): 3667
• Target Species: Human, Mouse, Rat, Monkey
• Isotype: IgG

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Concentration: 1 mg/ml
• Dilution: Western Blot 1:250-1:1500, ELISA 1:2000-1:10000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500
• Gene Accession No.: P35568, P35568, P35568, P35568
• Gene Symbols: IRS1
• Immunogen: This affinity purified IRS1 [p Ser307] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 290-320 of human IRS1 protein. (Uniprot: P35568)
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human IRS1 pS307 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with IRS1 from human, mouse, rat, dog and vervet monkey based on 100% homology with the immunizing sequence. Partial reactivity is also expected against IRS1 from pig (94%), bovine (94%) and chicken (88%) sources. Reactivity of this antibody with IRS1 from other species is unknown.
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

For Research Use Only