ISG15Rabbit anti-Human, Polyclonal, Rockland™

Catalog No.	Quantity
50-194-3804	500 μg

Catalog No. 50-194-3804 Supplier Rockland Immunochemicals Supplier No. 200401438

Description

Rabbit Polyclonal Antibody

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega , but not IFN-gamma . ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).

Specifications

• Antigen: ISG15
• Applications: ELISA, Immunohistochemistry, Western Blot
• Classification: Polyclonal
• Concentration: 5 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: ISG15
• Gene Accession No.: P05161
• Gene Alias: G1p2; hUCRP; IFI15; IGI15; IMD38; interferon, alpha-inducible protein; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; interferon-induced 15-KDa protein; Interferon-induced 17 kDa protein; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein (15 kDa); interferon-stimulated protein 15; interferon-stimulated protein, 15 kDa; IP17; Irfp; Isg15; ISG15 ubiquitin-like modifier; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15; Ucrp
• Gene Symbols: ISG15
• Host Species: Rabbit
• Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human ISG15 protein.
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 9636
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Lyophilized