Invitrogen™ ITCH Monoclonal Antibody (5E12)

Catalog No.	Quantity
PIMA549150	100 μg

Catalog No. PIMA549150 Supplier Invitrogen™ Supplier No. MA549150

Description

Mouse Monoclonal Antibody

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human HepG2 whole cell, human 293T whole cell, human PC-3 whole cell, rat brain tissue, mouse brain tissue. IHC: human renal clear cell carcinoma tissue, human breast cancer tissue, human liver cancer tissue. Flow: U937 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

Specifications

• Antigen: ITCH
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 5E12
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and no preservative
• Gene: ITCH
• Gene Accession No.: Q8C863, Q96J02
• Gene Alias: 6720481N21Rik; 8030492O04Rik; A130065M08; ADMFD; AIF4; AIP4; atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; C230047C07Rik; dJ468O1.1; E3 ubiquitin-protein ligase Itchy; E3 ubiquitin-protein ligase Itchy homolog; HECT-type E3 ubiquitin transferase Itchy homolog; ITCH; itch E3 ubiquitin ligase; itchy E3 ubiquitin protein ligase; itchy E3 ubiquitin protein ligase homolog; itchy, E3 ubiquitin protein ligase; NAPP1; NFE2-associated polypeptide 1
• Gene Symbols: ITCH
• Host Species: Mouse
• Immunogen: E.coli-derived human ITCH/AIP4 recombinant protein (Position: K17-E358).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 16396, 311567, 83737
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2b