Invitrogen™ ITGA2 Monoclonal Antibody (16B4)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA180784

Catalog No. PIMA180784

Description

This antibody does not react with rat. Sodium azide removal by dialysis is recommended prior to use in functional assays. Heat-mediated antigen retrieval using Tris/EDTA (pH 9) is recommended for the staining of paraffin sections. Mouse anti Human CD49b antibody, clone 16B4 recognizes human CD49b, also known as integrin α2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid approximately 160 kDa single pass type-1 transmembrane glycoprotein possessing multiple FG-GAP repeats and a single VWFA domain.

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

Specifications

• Antigen: ITGA2
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: P17301
• Gene Symbols: Itga2
• Immunogen: Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 16B4
• Conjugate: Unconjugated
• Gene: Itga2
• Gene Alias: alpha 2 subunit of VLA-2 receptor; BDPLT9; BR; CD49 antigen-like family member B; CD49B; Collagen receptor; DX5; GPIa; HPA-5; human platelet alloantigen system 5; integrin alpha 2; integrin alpha-2; integrin subunit alpha 2; integrin, alpha 2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); Itga2; platelet antigen Br; platelet glycoprotein GPIa; platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; VLA-2; VLA2 receptor; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLAA2
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 3673
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid