Invitrogen™ ITGA4 Monoclonal Antibody (Bu49)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA181535

Catalog No. PIMA181535

Description

The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is human tonsil. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti Human CD49d monoclonal antibody, clone Bu49 recognizes human CD49d, also known as integrin alpha 4 or VLA-4 alpha subunit. CD49d is a approximately 150 kDa single pass type I transmembrane glycoprotein.

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

Specifications

• Antigen: ITGA4
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: P13612
• Gene Symbols: ITGA4
• Immunogen: Hyatt myeloma cell line.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human, Canine
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
• Clone: Bu49
• Conjugate: Unconjugated
• Gene: ITGA4
• Gene Alias: 269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; IA4; integrin alpha 4; integrin alpha-4; integrin alpha4B; Integrin alpha-IV; integrin subunit alpha 4; integrin, alpha 4; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); Itga4; Itga4B; ITGA-IV; LPAM subunit alpha; lymphocyte Peyer patch adhesion molecules subunit alpha; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 3676, 488429
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid