Invitrogen™ ITGA4 Monoclonal Antibody (PS/2)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA182769

Catalog No. PIMA182769

Description

This product is a low-endotoxin, preservative-free formulation. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Rat anti Mouse CD49d monoclonal antibody, clone PS/2 recognizes murine alpha 4 integrin (CD49d), a approximately 150 kDa single pass type I membrane glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively (Holzmann et al. 1989).

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

Specifications

• Antigen: ITGA4
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.4
• Gene Accession No.: P13612, Q00651
• Gene Symbols: ITGA4
• Immunogen: P815 DBA/2 murine mastocytoma cells.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation
• Clone: PS/2
• Conjugate: Unconjugated
• Gene: ITGA4
• Gene Alias: 269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; IA4; integrin alpha 4; integrin alpha-4; integrin alpha4B; Integrin alpha-IV; integrin subunit alpha 4; integrin, alpha 4; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); Itga4; Itga4B; ITGA-IV; LPAM subunit alpha; lymphocyte Peyer patch adhesion molecules subunit alpha; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 16401, 3676
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov