Invitrogen™ ITGB1 Monoclonal Antibody (HM beta 1-1)

Armenian Hamster Monoclonal Antibody

Supplier:  Invitrogen™ MA181498

Catalog No. PIMA181498

Description

For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Hamster anti Mouse CD29 antibody, clone HM beta 1-1 recognizes the murine integrin beta 1 subunit (CD29), a approximately 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes.

CD29, also known as ITGB1 or Integrin Subunit Beta 1, is a 110 kDa cell surface glycoprotein widely expressed by various cells, including all leukocytes. It forms non-covalently linked heterodimers with at least six different alpha integrins (CD49a-f), creating VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers mediate a range of cellular responses, including adhesion, trafficking, proliferation, and differentiation. CD29-containing integrins bind to extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin. Some integrins also interact with cellular receptors like VCAM-1 and MadCAM-1. CD29 plays a crucial role in immune cell trafficking from blood to tissue, particularly during leukocyte emigration and extravascular migration. It is involved in regulating tissue damage and disease symptoms related to inflammatory bowel disease. In the presence of anti-tumor drugs, decreased expression of ITGB1 correlates with multidrug resistance in tumor cells. ITGB1-dependent mechanisms enhance cytokine secretion in human airway smooth muscle in response to IL-1beta, facilitated by fibronectin and type I collagen. More than eight beta subunits with numerous splice variant isoforms have been identified in mammals, including two major forms: beta1A and beta1D. Beta1A is present in all tissues except cardiac and skeletal muscle, which express the beta1D variant.

Specifications

• Antigen: ITGB1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: P09055, P49134
• Gene Symbols: ITGB1
• Immunogen: Purified murine VLA-4 antigen.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Immunoprecipitation
• Clone: HM beta 1-1
• Conjugate: Unconjugated
• Gene: ITGB1
• Gene Alias: 4633401G24Rik; AA409975; AA960159; Beta OL; beta oligodendroglia; CD29; CSAT antigen; ENSMUSG00000051907; Fibronectin receptor subunit beta; FNRB; Glycoprotein IIa; Gm9863; gpIIa; integrin beta 1; integrin beta 1 (fibronectin receptor beta); integrin beta 1A precursor; Integrin beta1; integrin beta-1; integrin beta-1 subunit; integrin beta1D; integrin precursor; integrin subunit beta 1; integrin VLA-4 beta subunit; integrin VLA-4 subunit beta; integrin, beta 1; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITBG1D; ITGB1; JG22 antigen; MDF2; MSK12; RGD-receptor; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLAB; VLA-BETA
• Host Species: Armenian Hamster
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 16412, 24511
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid