Tocris Bioscience™ Janelia Fluor 635, SE

Description

Red dye (Reactive group: NHS ester). Applications: super resolution microscopy, confocal, flow and live cell imaging

Janelia Fluor® 635, SE is a red fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag™ and SNAP-tag™. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Also suitable for flow cytometry. Cell permeable. Excitation maximum = 635 nm; emission maximum = 652 nm; Quantum yield = 0.56; Max extinction coefficient = 167,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0524. Exhibits low nonspecific background staining.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor™ conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Specifications

• Chemical Name or Material: 1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-7-[1-(3-fluoroazetidinyl)]-9-silaanthracen-2(9 H)-ylidene]-3-fluoroazetidinium, inner salt
• Quantity: 2 mg
• Target: Amine-Reactive Janelia Fluor™ Dyes
• Molecular Formula: C₃₃H₂₉F₂N₃O₆Si
• Formula Weight: Observed MW: 629.69