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Tocris Bioscience™ Janelia Fluor™ 635b, NHS ester
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Catalog No. 8157200U Shop All R&D Systems Products
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81-572-00U 200 μg
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Catalog No. 81-572-00U Supplier Tocris Bioscience™ Supplier No. 8157/200U
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Description

Blinking fluorescent dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM) including SMLM

Spontaneously Blinking Janelia Fluor™ Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor™ technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.

Key Information: Janelia Fluor™ 635b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines.

Used for: suitable for DNA labelling using amine-terminated oligonucleotides. Also used to label oligonucleotides for super-resolution assays of transposase-accessible chromatin (ATAC-SMLM).

Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).

Properties and Photophysical Data: derived from Janelia Fluor™ 635, SE (Cat. No. 6419). Excitation and emission maxima (λ) are 654 nM and 671 nM, respectively.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

Specifications

Chemical Name or Material 2,5-Dioxopyrrolidin-1-yl 3,7-bis(3-fluoroazetidin-1-yl)-5,5-dimethyl-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxylate
Quantity 200 μg
Target Amine-Reactive Janelia Fluor Dyes
Molecular Formula C33H31F2N3O5Si
Purity 0.9
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