Tocris Bioscience™ Janelia Fluor 646, Maleimide

Catalog No. 65-902

Supplier: Tocris Bioscience™ 6590/2

Description

Red dye (Reactive group: maleimide). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging

Janelia Fluor® 646, Maleimide is a red fluorogenic fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two color imaging with Janelia Fluor™ 549 SE (Cat. No. 6147). Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54; Max. extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor is 0.19.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor™ conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

Specifications

• Chemical Name or Material: 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1 H-pyrrol-1-yl)ethyl)carbamoyl)benzoate
• Quantity: 2 mg
• Target: Sulfhydyl-Reactive Janelia Fluor™ Dyes
• Molecular Formula: C₃₅H₃₄N₄O₅Si
• Formula Weight: 618.75
• Purity: 95%