Tocris Bioscience™ JFX™ 650, NHS ester

Description

Deuterated Janelia Fluor dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging

Key Information: JFX™ 650, NHS ester is a red fluorescent deuterated Janelia Fluor™ dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.

Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: JFX™ 650, NHS ester shows enhanced brightness, photostability and chromostability. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag™ and SNAP-tag™. Excitation and emission maxima (λ) are 650 nm and 667 nm, respectively; quantum yield = 0.53; extinction coefficient = 17,600 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.015.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We also offer Janelia Fluor™ conjugated antibodies and custom conjugation services with our sister company Novus Biologicals Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

JFX is a trademark owned by the Howard Hughes Medical Institute.

Specifications

• Chemical Name or Material: 2-(5,5-Dimethyl-3-(pyrrolidin-1-ium-1-ylidene-d8)-7-(pyrrolidin-1-yl-d8)-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate
• CAS: 2396479-84-6
• Quantity: 200 μg
• Target: Amine-Reactive Janelia Fluor Dyes
• Molecular Formula: C35H19D16N3O6Si
• Purity: 0.9