Invitrogen™ JMJD3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA532192

Catalog No. PIPA532192

Description

Recommended positive controls: 293T, A375. Predicted reactivity: Mouse (98%), Rat (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Specifications

• Antigen: JMJD3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: KDM6B
• Gene Alias: 1700064E03Rik; BC038313; jmjC domain-containing protein 3; JMJD3; jumonji domain containing 3; jumonji domain containing 3, histone lysine demethylase; jumonji domain-containing 3; jumonji domain-containing protein 3; KDM1 lysine (K)-specific demethylase 6B; Kdm6b; KIAA0346; lysine (K)-specific demethylase 6B; lysine demethylase 6B; Lysine-specific demethylase 6B; mKIAA0346
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23135
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.95 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: O15054
• Gene Symbols: KDM6B
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1100 and 1358 of Human JMJD3.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG