Invitrogen™ JMY Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143713

Catalog No. PIPA5143713

Description

The antibody detects a 130 kDa* protein corresponding to the molecular mass of JMY on SDS-PAGE immunoblots of human HeLa, Jurkat cells, rat PC12 cells, and mouse brain and heart.

JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.

Specifications

• Antigen: JMY
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: JMY
• Gene Alias: FLJ37870; JMY; junction mediating and regulatory protein, p53 cofactor; junction-mediating and regulatory protein; junction-mediating and -regulatory protein; LOC683593; p300 transcriptional cofactor JMY; similar to junction-mediating and regulatory protein; WAS protein homology region 2 domain containing 1-like 3; WHAMM2; WHDC1L3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 133746, 57748, 683593
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: Q8N9B5, Q9QXM1
• Gene Symbols: JMY
• Immunogen: JMY synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of mouse JMY. This sequence is highly conserved in human, rat, frog, and zebrafish JMY proteins, and has low homology to other WASP family members.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG