Kappa Light Chain Antibody (KLC709), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP234259F	Novus Biologicals NBP2342590.02MG	0.02 mg
NBP234259A	Novus Biologicals NBP2342590.1MG	0.1 mg
NBP234259B	Novus Biologicals NBP2342590.2MG	0.2 mg

Description

Kappa Light Chain Monoclonal specifically detects Kappa Light Chain in Human samples. It is validated for Simple Western, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array.

Specifications

• Antigen: Kappa Light Chain
• Dilution: Simple Western 10 ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 0.5-1ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P01601
• Gene ID (Entrez): 3514
• Immunogen: Recombinant human Ig kappa chain
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 22.5 kDa

• Clone: KLC709
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Immunology
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
• Gene Symbols: IGKC
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. It recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.