Invitrogen™ KCC2 Monoclonal Antibody (N1/12)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA527610

Catalog No. PIMA527610

Description

1 μg/mL of MA5-27610 was sufficient for detection of KCC2 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 140kDa. This antibody was formerly sold as clone S1-12.

Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.

Specifications

• Antigen: KCC2
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Gene Accession No.: Q63633, Q91V14, Q9H2X9
• Gene Symbols: SLC12A5
• Immunogen: Fusion protein amino acids 932-1043 corresponding to rat KCC2.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: N1/12
• Conjugate: Unconjugated
• Gene: SLC12A5
• Gene Alias: EIEE34; EIG14; Electroneutral potassium-chloride cotransporter 2; erythroid K-Cl cotransporter 2; furosemide-sensitive K-Cl cotransporter; hKCC2; KCC 2; Kcc2; KCC2a variant 1; KCC2a-S25 variant 1; KCC2b variant 2; K-Cl cotransporter 2; KIAA1176; mKCC2; mKIAA1176; neuronal K-Cl cotransporter; neuronal-specific K-Cl cotransporter; rKCC2; S12A5; SLC12A5; solute carrier family 12 (potassium/chloride transporter), member 5; solute carrier family 12 (potassium-chloride transporter), member 5; solute carrier family 12 member 5; solute carrier family 12, (potassium-chloride transporter) member 5; solute carrier family 12, member 5
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 171373, 57138, 57468
• Content And Storage: -20°C
• Form: Liquid