Invitrogen™ KCNMA1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1923

Catalog No. PA1923

Description

PA1-923 detects large conductance, calcium-activated potassium channel alpha (Maxi K+ alpha) from human tissues as well as recombinant human protein and rat samples. PA1-923 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~110 kDa protein representing Maxi K+ alpha from human myometrial smooth muscle cell extracts. Immunocytochemical staining of Maxi K+ alpha in human coronary muscle cells with PA1-923 results in predominant staining of the plasma membrane. PA1-923 immunizing peptide corresponds to amino acid residues 945-961 from human Maxi K+ alpha. This sequence is completely conserved across many species. PA1-923 immunizing peptide (Cat. # PEP-113) is available for use in neutralization and control experiments.

Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.

Specifications

• Antigen: KCNMA1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Kcnma1
• Gene Alias: 5730414M22Rik; bA205K10.1; big potassium channel alpha subunit; BK channel; BK channel alpha subunit; BKCa; BKCA alpha; BKCA alpha subunit; BKTM; calcium-activated potassium channel alpha subunit; calcium-activated potassium channel subunit alpha-1; calcium-activated potassium channel subunit alpha-1; uncharacterized protein; calcium-activated potassium channel, subfamily M subunit alpha-1; hSlo; K(VCA)alpha; KCa1.1; Kcnma; Kcnma1; KCNMA1b; KCNMA1c; Maxi K channel; maxiK; maxi-K channel HSLO; mSlo; mSlo1; potassium calcium-activated channel subfamily M alpha 1; potassium channel, calcium activated large conductance subfamily M alpha, member 1; potassium large conductance calcium-activated channel, subfamily M, alpha member 1; SAKCA; SLO; slo homolog; slo1; Slo-alpha; Slowpoke homolog; stretch-activated Kca channel
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3778, 83731
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: Q12791, Q62976
• Gene Symbols: Kcnma1
• Immunogen: Synthetic peptide corresponding to residues C T(945) E L V N D T N V Q F L D Q D D D(961).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG