Ketohexokinase Isoform C, Mouse anti-Human, Clone: 5F1-3F5, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABS1907100UG

Catalog No. MABS1907100

Description

Anti-Ketohexokinase Isoform C, clone 5F1-3F5, Cat. No. MABS1907, is a mouse monoclonal antibody that detectsKetohexokinase and has been tested for use in Western Blotting.

Ketohexokinase (UniProt: P50053; also known as EC: 2.7.1.3, Hepatic fructokinase) is encoded by the KHK gene (Gene ID: 3795) in human. Ketohexokinase is a homodimeric cytosolic enzyme in that catalyzes the conversion of fructose to fructose 1 phosphate. It is most abundant in the liver, where it may constitute up to 0.6% of the total liver protein. It has also been reported in kidney, spleen, gut, and pancreas. Very low levels are also found in adrenal, muscle, brain, and eyes. It has multiple fructose binding sites. There are two major isoforms of this enzyme ketofructokinase C and ketofructokinase A. Due to its high Km (∽ 28 mM), fructose is preferentially metabolized by the C isoform. Ketohexokinase -C is unique among sugar kinases in that its metabolism of fructose is associated with a rapid depletion of intracellular ATP. Unlike glucokinase, in which excessive phosphorylation of glucose is prevented by a negative feedback system, the metabolism of fructose by ketohexokinase-C results in rapid phosphorylation with a fall in intracellular phosphate and ATP. If the ATP levels fall significantly then the protein synthesis is transiently stopped and cells may develop features that are consistent with ischemia. Mutations in KHK gene has been linked to fructosuria, a clinically benign condition that is characterized nu the incomplete metabolism of fructose in the liver, leading to its urinary excretion. (Ref.: Diggle, CP et al., (2009). J. Histochem. Cytochem. 57(8): 763-774; Hayward, BE., and Bonthron, DT (1998). Eur. J. Biochem. 257(1); 85-91).

Specifications

• Antigen: Ketohexokinase Isoform C
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: KHK
• Immunogen: A full-length human recombinant Ketohexokinase, isoform C.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_000212
• Target Species: Human
• Form: Purified

• Applications: Western Blot
• Clone: 5F1-3F5
• Dilution: Western Blotting Analysis: 2μg/mL from a represenative lot detected Ketohexokinase Isoform C in HEK293 cells expressing HA-tagged KHK-C.Western Blotting Analysis: 1μg/mL from a represenative lot detected Ketohexokinase Isoform C in human liver tissue lysate.
• Gene Alias: EC: 2.7.1.3;Hepatic fructokinase;Fructokinase
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 5F1-3F5 specifically detects isoform C of human ketohexokinase.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG1 κ