Invitrogen™ Ki-67 Monoclonal Antibody (Ki-67), Alexa Fluor™ 488

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA544124

Catalog No. PIMA544124

Description

This antibody recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

Specifications

• Antigen: Ki-67
• Classification: Monoclonal
• Conjugate: Alexa Fluor 488
• Gene: Mki67
• Gene Alias: antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05
• Host Species: Mouse
• Purification Method: Size-exclusion Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4288, 513220
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: Ki-67
• Formulation: PBS with 0.2% BSA and 15mM sodium azide
• Gene Accession No.: P46013
• Gene Symbols: Mki67
• Immunogen: Nuclei of the Hodgkin lymphoma cell line L428.
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human, Bovine
• Product Type: Antibody
• Isotype: IgG1

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov