Ki-67 Monoclonal Antibody (SolA15), PE-Cyanine5, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
15-569-882	Invitrogen™ 15569882	100 μg

Description

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the Ki-67 labeling index refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This SolA15 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523-00) and protocol. Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins located at thermofisher.com. This may be used at less than or equal to 0.5 μg per test.

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

Specifications

• Antigen: Ki-67
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: E9PVX6, P46013
• Gene Symbols: Mki67
• Primary or Secondary: Primary
• Product Type: Antibody

• Clone: SolA15
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine5
• Host Species: Rat
• Target Species: Human, Mouse, Rat, Canine, Monkey, Cynomolgus Monkey
• Gene ID (Entrez): 100686578, 102135895, 17345, 291234, 4288
• Isotype: IgG2a κ
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!