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Ki-67 Rat anti-Canine, Cynomolgus Monkey, Human, Mouse, Non-human primate, Rat, eFluor® 615, Clone: SolA15, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 42569880

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Catalog No. 50-113-0719


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Description

Description

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. Applications Reported: This SolA15 antibody has been reported for use in immunocytochemistry and immunohistochemistry. Applications Tested: This SolA15 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells at less than or equal to 0.5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength.

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the Ki-67 labeling index refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.Applications Reported: This SolA15 antibody has been reported for use in immunocytochemistry and immunohistochemistry.Applications Tested: This SolA15 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells at less than or equal to 0.5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength. (for example Excitation 560/55, 585LP, Emission 645/75) A standard Alexa Fluor™ 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2µm post-manufacturing filtered.
Specifications

Specifications

Ki-67
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P46013, E9PVX6
antigen identified by monoclonal antibody Ki-67, antigen KI-67, KIA, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105, RP11-380J17.2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Canine, Cynomolgus Monkey, Human, Murine, Non-human Primate, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
SolA15
eFluor 615
MKI67
Liquid
MKI67
IgG2a, kappa, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
100686578, 102135895, 17345, 291234, 4288
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